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      • Unit 731 - Guide >
        • Background of Biochemical Warfare Development
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        • Map of Unit 731
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        • Duties of Unit 731
        • Human Experimentation
        • [GRAPHIC] Germ Warfare Attacks
        • Cover Ups After the War
        • [OLD] Cover Ups After the War
      • Philippines' Resistance - Guide >
        • Philippines World War II Timeline
        • The Japanese Invasion & Conquest of the Philippines
        • Bataan Death March
        • Formation of Underground Philippines Resistance
        • Supplies of the Guerrilla Fighters
        • The Hukbalahap
        • Hunter's ROTC
        • Marking's Guerrillas
        • United States Army Forces in the Philippines of Northern Luzon (USAFIP-NL)
        • The Aetas
        • Chinese and Filipino-Chinese Nationalist Guerrilla Units
        • The Female Faces of the Philippine Guerrillas
      • Rising Sun Flag - Guide >
        • History of the Rising Sun Flag
        • Atrocities Committed Under the Flag
        • Rising Sun Flag in Pop Culture
      • Pinay Guerrilleras - Guide >
        • Japanese Occupation of the Philippine Islands: Pinays Answering the Call to Arms
        • The Fierce Heneralas and Kumanders of the Hukbalahap Guerrillas
        • Amazons of the Pacific Theater
        • Filipina American Veterans: Recovering the Extraordinary Feats of the Ordinary Pinays
        • The Legacy of the Asian Women Soldier
      • Fall of Singapore - Guide >
        • Singapore World War II Timeline
        • History of World War II in the Pacific
        • History of Singapore
        • Japan's Conquest in Asia
        • Japan's Invasion of the Malay Peninsula
        • Sook Ching Massacre
        • Double Tenth Incident
        • Social Changes and Challenges in Singapore
        • Voices from Syonan
        • Return to British Rule
      • Three Years and Eight Months - Guide >
        • Hong Kong before WW2
        • Buildup to World War 2
        • The Battle of Hong Kong
        • Life during 3 Years and 8 Months
        • East River Column Guerrilla Fighters
        • Prisoners of War Camps
        • End of Japanese Occupation
        • War Crimes Trials
      • Siamese Sovereignty - Guide >
        • The Land of Smiles
        • The Thai-Japanese Relationship
        • Phibun’s Domestic and International Policies
        • The Free Thai Resistance Movement
        • Post WW2 Aftermath of Thailand
      • The Khabarovsk War Crimes Trial - Guide >
        • Defendants of Khabarovsk War Crime
        • The Japanese Empire and USSR in WW2
        • The Employment of the Bacteriological Weapon in the War
        • Planning of Japan invasion to USSR
      • Unit 731 Cover-up : The Operation Paperclip of the East - Guide >
        • Establishing Manchukuo
        • The Development of Unit 731
        • Plan Kantokuen and Bacteriological Warfare
        • The Downfall of the Japanese WW2 Era
        • Three Stages of Interrogations
        • Lasting Impacts
      • Marutas of Unit 731 - Guide >
        • How did Ishii Shiro start unit 731?
        • A Beta Testing Site
        • Establishing Pingfan
        • Experiences at the Human Experimentation Complex
        • Vivisection at the Unit 731
        • Anta Testing Grounds
        • Overall Advance from the Laboratory Creations
        • The End of the War
      • Prince Konoe Memoir - Guide >
        • Who is Prince Konoe?
        • Preparation to Tripartite Pact
        • Emperor Hirohito and Prince Konoe
        • The End of Prince Konoe
      • Competing Empires in Burma - Guide >
        • What was the China-Burma-India Theater?
        • When did the China-Burma-India Theater Happen?
        • Who Fought in the China-Burma-India Theater?
        • The Second Sino Japanese War
        • Japan in the South
        • Operation U-Go
      • Battle of Shanghai - Guide >
        • The Battle of Shanghai. Background
        • Shanghai Before War
        • The First Battle of Shanghai 1932
        • Battle of Shanghai 1937
        • Aftermath of Battle for Shanghai
      • Ishi Shiro - Guide >
        • History of Biological Weapons and The Young Ishii Shiro
        • Establishment in Manchuria
        • Pingfang District - Harbin
        • Failures and Corruption
        • Post War
      • Taiwan The Israel of the East - Guide >
        • Background of Formosa
        • Industrialization of Japan
        • China During WWII
        • Taiwan under Kuomintang
        • New Taiwanese National Identity
      • Seeking Justice for Biological Warfare Victims of Unit 731 - Guide >
        • Introduction of Wang Xuan
        • Colonel Memorandum
        • The Beginning of Biological Warfare
        • The Bacteriological Warfare on China
        • Victims in Zhejiang’s Testimonies
        • After the War
      • Rice and Revolution - Guide >
        • The French Colonial Period
        • Anti-Colonial Resistance
        • The Rise of the Communist Movement
        • Imperial Japan’s Entry into Indochina
        • The Portents of Famine
        • The Famine (1944-45)
        • Legacy of the 1944-45 Vietnam Famine
      • Clash of Empires - Guide >
        • Japan’s Imperialist Origins
        • Japan’s Competition against the West: Nanshin-ron and Hokushin-ron
        • Japanese Imperialism Through the Lens of French Indochina
        • The U.S.-Japan Relations and the Pearl Harbor Attack
      • Hunger for Power and Self-SufficiencyI - Guide >
        • The Influence of War Rations on Post-War Culinary Transformations
        • How World War II Complicated Food Scarcity and Invention
        • American Military Innovations
        • Government-Sponsored Food Inventions in Europe during World War II
        • Feeding the Army: The Adaptation of Japanese Military Cuisine and Its Impact on the Philippines
        • Mixed Dishes: Culinary Innovations Driven by Necessity and Food Scarcity
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      • Scientific Advancements
      • Seeking Justice: A Humanities Lesson Plan
      • The Hukbalahap
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Human Experimentation at Unit 731

Unit 731 is infamous for its human experimentation during its existence during World War 2. At least 3,000 men, women, and children were subjected as "marutas" or as logs to experimentations conducted by Unit 731 division at Pingfang alone. Here is an article on why they were called "marutas". Dr. Harris Sheldon estimates that at least 10,000 to 12,000 prisoners died in the biological experiments. 

Frostb​ite Testing

​Yoshimura Hisato, a physiologist in Unit 731, had a special interest in hypothermia and used human subjects to test human's reactions to frostbites. Hisato routinely submerged prisoner's limbs in a tub of water filled with ice and held them there until the limbs were frozen solid and a coat of ice were formed over the skin. He timed the victims to check how long it took for the human bodies to develop frost bites. According to one of the witness to the frostbite testing, the limbs made a sound like a plank of wood when struck with a cane. Then he tried different methods for rapidly thawing of the frozen appendage such as dousing limbs with hot water, open fire, or leaving the subject untreated overnight to see how long it took for the prisoner's blood to thaw it out. Unit 731 was able to prove scientifically that the best treatment for frostbite was to immerse it in water a bit warmer than 100 degrees but never more than 122 degrees. 

Warning: Graphic Image 

Picture of Frostbite Experimentation

Vivisection of Conscious Prisoners​

Picture
General Shiro Ishii performing vivisection on a prisoner of Unit 731. 
Vivisection is the practice of performing operations on living beings for the purpose of experimentation or scientific research. Vivisection was performed in Unit 731 without anesthesia to study the operations of living systems. It was performed on thousands of victims, mostly Chinese communist prisoners as well as children and elderly farmers. They were infected with diseases such as cholera and the plague, and then had their organs removed for examination before they died in order to study the effects of the disease without decomposition after death. 
Subjects that were used to study the progress of gangrene had their limbs amputated and reattached to the other side of the body while others had their limbs crushed or frozen, or had their circulation cut off. 
After the body was used up and exhausted, they were normally shot or killed by lethal injection. 

Weapon Testing

Unit 731 studied bayonets, swords, and knives with the use of their prisoners. They also studied flamethrowers on both covered and exposed skin. They also set up gas chambers to test subjects with blister agents and nerve gas. They also studied prolonged X-ray exposure, which sterilized and killed thousands of testing subjects. 

Testing the Human Limits

There were human subjects locked up and deprived of food and water to learn how long humans could survive.  In order to study crash injuries, heavy subjects were dropped onto bounded prisoners. The effects of high G-forces on pilots and falling paratroopers were studied by loading human beings into large centrifuges and spun them at higher and higher speeds until they lost consciousness or died, which usually happened between 10-15 G's. 

Venereal Disease

The Imperial Japanese Army was interested in the symptoms and treatment of syphilis. Male prisoners infected with syphilis were ordered to rape female and male prisoners to monitor the onset of the disease. More rapes were arranged until the exposure establish infection. 

Rape and Forces Pregnancy

Female prisoners of childbearing age were forcibly impregnated so that weapon and trauma experiments could be done on them. Pregnant test subjects were infected with various diseases, exposed to chemical weapons, crash injuries, bullet wounds, and shrapnel injuries. Then they were opened up and the effects on the fetuses were studied. Pregnant women with syphilis was of special interests to the researchers of Unit 731.


This content is a supplementary resource guide to our ebook.

​

Picture

Unit 731- The Forgotten Asian Auschwitz

The Japanese invasion of China during the Second Sino-Japanese war has left a strong legacy of hate and disgust among many Chinese today. Much of the atrocities committed by the Japanese are now known to most historians. By far, the most despicable and forgotten act against humanity committed by the Imperial Japanese government was its covert biochemical weapons program. Euphemistically labelled as the "Epidemic Prevention and Water Purification Department" of the Imperial Japanese Army, the Japanese conducted a wide range of cruel and inhumane experiments on prisoners who were often innocent. ​
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​Duties of Unit 731

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Germ Warfare Attacks

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  • Home
    • About >
      • FAQ's - Frequently Asked Questions
    • Support Us >
      • Projects you can support! >
        • Distributing Books
        • Presenting at 112th Annual Meeting of Pacific Coast Branch
        • Summer Research Relocation Fund
    • Contact
  • Stories
    • Videos >
      • Black Hearts (2021)
    • Blog
    • Podcast: Forgotten History
  • Internship
    • Summer 2023 Internship
    • Fall 2022 Internship
    • Summer 2022 Internship
    • Summer 2021 Internship
    • Fall 2020- Spring 2021 Internship
    • Summer 2020 Internship
    • Fall 2019 Internship
    • Summer 2019 Internship >
      • Public History Night
    • School Year 2018-2019 Internship
    • Summer 2018 Internship >
      • 2018 Summer Showcase + Fundraiser
    • Fall 2017 Internship
    • Summer 2017 Internship >
      • 2017 Summer Showcase & Fundraiser
  • Books
  • Archives
  • Resource Page
    • Supplementary Research Guides >
      • Unit 731 - Guide >
        • Background of Biochemical Warfare Development
        • Imperial Japan's Chemical Warfare Development Program
        • Map of Unit 731
        • Personnel of Unit 731
        • Duties of Unit 731
        • Human Experimentation
        • [GRAPHIC] Germ Warfare Attacks
        • Cover Ups After the War
        • [OLD] Cover Ups After the War
      • Philippines' Resistance - Guide >
        • Philippines World War II Timeline
        • The Japanese Invasion & Conquest of the Philippines
        • Bataan Death March
        • Formation of Underground Philippines Resistance
        • Supplies of the Guerrilla Fighters
        • The Hukbalahap
        • Hunter's ROTC
        • Marking's Guerrillas
        • United States Army Forces in the Philippines of Northern Luzon (USAFIP-NL)
        • The Aetas
        • Chinese and Filipino-Chinese Nationalist Guerrilla Units
        • The Female Faces of the Philippine Guerrillas
      • Rising Sun Flag - Guide >
        • History of the Rising Sun Flag
        • Atrocities Committed Under the Flag
        • Rising Sun Flag in Pop Culture
      • Pinay Guerrilleras - Guide >
        • Japanese Occupation of the Philippine Islands: Pinays Answering the Call to Arms
        • The Fierce Heneralas and Kumanders of the Hukbalahap Guerrillas
        • Amazons of the Pacific Theater
        • Filipina American Veterans: Recovering the Extraordinary Feats of the Ordinary Pinays
        • The Legacy of the Asian Women Soldier
      • Fall of Singapore - Guide >
        • Singapore World War II Timeline
        • History of World War II in the Pacific
        • History of Singapore
        • Japan's Conquest in Asia
        • Japan's Invasion of the Malay Peninsula
        • Sook Ching Massacre
        • Double Tenth Incident
        • Social Changes and Challenges in Singapore
        • Voices from Syonan
        • Return to British Rule
      • Three Years and Eight Months - Guide >
        • Hong Kong before WW2
        • Buildup to World War 2
        • The Battle of Hong Kong
        • Life during 3 Years and 8 Months
        • East River Column Guerrilla Fighters
        • Prisoners of War Camps
        • End of Japanese Occupation
        • War Crimes Trials
      • Siamese Sovereignty - Guide >
        • The Land of Smiles
        • The Thai-Japanese Relationship
        • Phibun’s Domestic and International Policies
        • The Free Thai Resistance Movement
        • Post WW2 Aftermath of Thailand
      • The Khabarovsk War Crimes Trial - Guide >
        • Defendants of Khabarovsk War Crime
        • The Japanese Empire and USSR in WW2
        • The Employment of the Bacteriological Weapon in the War
        • Planning of Japan invasion to USSR
      • Unit 731 Cover-up : The Operation Paperclip of the East - Guide >
        • Establishing Manchukuo
        • The Development of Unit 731
        • Plan Kantokuen and Bacteriological Warfare
        • The Downfall of the Japanese WW2 Era
        • Three Stages of Interrogations
        • Lasting Impacts
      • Marutas of Unit 731 - Guide >
        • How did Ishii Shiro start unit 731?
        • A Beta Testing Site
        • Establishing Pingfan
        • Experiences at the Human Experimentation Complex
        • Vivisection at the Unit 731
        • Anta Testing Grounds
        • Overall Advance from the Laboratory Creations
        • The End of the War
      • Prince Konoe Memoir - Guide >
        • Who is Prince Konoe?
        • Preparation to Tripartite Pact
        • Emperor Hirohito and Prince Konoe
        • The End of Prince Konoe
      • Competing Empires in Burma - Guide >
        • What was the China-Burma-India Theater?
        • When did the China-Burma-India Theater Happen?
        • Who Fought in the China-Burma-India Theater?
        • The Second Sino Japanese War
        • Japan in the South
        • Operation U-Go
      • Battle of Shanghai - Guide >
        • The Battle of Shanghai. Background
        • Shanghai Before War
        • The First Battle of Shanghai 1932
        • Battle of Shanghai 1937
        • Aftermath of Battle for Shanghai
      • Ishi Shiro - Guide >
        • History of Biological Weapons and The Young Ishii Shiro
        • Establishment in Manchuria
        • Pingfang District - Harbin
        • Failures and Corruption
        • Post War
      • Taiwan The Israel of the East - Guide >
        • Background of Formosa
        • Industrialization of Japan
        • China During WWII
        • Taiwan under Kuomintang
        • New Taiwanese National Identity
      • Seeking Justice for Biological Warfare Victims of Unit 731 - Guide >
        • Introduction of Wang Xuan
        • Colonel Memorandum
        • The Beginning of Biological Warfare
        • The Bacteriological Warfare on China
        • Victims in Zhejiang’s Testimonies
        • After the War
      • Rice and Revolution - Guide >
        • The French Colonial Period
        • Anti-Colonial Resistance
        • The Rise of the Communist Movement
        • Imperial Japan’s Entry into Indochina
        • The Portents of Famine
        • The Famine (1944-45)
        • Legacy of the 1944-45 Vietnam Famine
      • Clash of Empires - Guide >
        • Japan’s Imperialist Origins
        • Japan’s Competition against the West: Nanshin-ron and Hokushin-ron
        • Japanese Imperialism Through the Lens of French Indochina
        • The U.S.-Japan Relations and the Pearl Harbor Attack
      • Hunger for Power and Self-SufficiencyI - Guide >
        • The Influence of War Rations on Post-War Culinary Transformations
        • How World War II Complicated Food Scarcity and Invention
        • American Military Innovations
        • Government-Sponsored Food Inventions in Europe during World War II
        • Feeding the Army: The Adaptation of Japanese Military Cuisine and Its Impact on the Philippines
        • Mixed Dishes: Culinary Innovations Driven by Necessity and Food Scarcity
    • Lesson Plans >
      • Reparations
      • Ethics in Science
      • Writing the Narrative of a Pinay Fighter
      • Privilege Journal
      • Environmental Injustices
      • Female Guerrillas
      • Hunter's ROTC
      • Scientific Advancements
      • Seeking Justice: A Humanities Lesson Plan
      • The Hukbalahap
      • Trading Immunity
      • Bataan Death March
      • Biochemical Warfare Development
Contribute